Step 1Allow the test cup, specimen, and/or controls to reach room temperature (15-30°C) prior to testing. Instruct the donor to not place anything in the mouth including food, drink, gum or tobacco products for at least 10 minutes prior to collection.
Step 2Remove the collection sponge and test cube from the sealed pouch, and tear off the packaging around the collection sponge.
Step 3Insert the sponge end of the saliva collector into the mouth. Actively swab the inside of the mouth and tongue to collect oral fluid for 2-3 minutes - until the sponge becomes fully saturated. Gentle pressing of the sponge between the tongue and teeth will assist saturation.
Step 4Remove the collector from the mouth. Place saturated oral fluid collector into test cup and screw the collector to press the sponge fully to release oral fluid.
Step 5Place the test cup on a clean and level surface. Remove the peel-off label, wait for the flow to appear in test windows and start a timer.
Step 6If the sample does not migrate in the test cup after 3 minutes, rotate the cup 4-5 times.
Step 7Read the test results after 3-10 minutes. If all lines are clearly visible at 3 minutes or sooner, then the test can be interpreted as negative and discarded. If any lines are not visible at 3 minutes, then the test should be re-read at 10 minutes.
Step 8For the alc strip, when applicable, the results should be read at 2 minutes. Compare the colour of the reaction pad with the chart provided on foil pouch to determine the relative oral fluid alc level.